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目的 在牛蛙背根神经节神经元研究P物质对γ -氨基丁酸激活电流的调制作用。方法 实验采用的方法是全细胞膜片钳技术 ,实验材料为急性分离的牛蛙背根神经节神经元。结果 在受检的神经元中大多数神经元 ( 95% )对γ -氨基丁酸 ( 1～ 1 0 0 0um)敏感 ,并且能被荷包牡丹碱 (GABA受体的阻断剂 )所阻断。另一方面 ,P物质能抑制γ -氨基丁酸激活电流的 3 0 .85%。结论 由于P物质受体和γ -氨基丁酸受体共存于牛蛙DGR神经元膜 ,并且P物质受体的激活可以调制γ -氨基丁酸受体激活的反应 ,从背角神经元释放的P物质可以逆转由γ -氨基丁酸介导的突触前抑制反应
Objective To study the effect of substance P on the modulation of γ - aminobutyric acid - activated current in dorsal root ganglion neurons of. Methods The whole cell patch clamp technique was used in the experiment. The experimental materials were acutely isolated bullfrog dorsal root ganglion neurons. Results Most of the neurons in the neurons examined (95%) were sensitive to γ - aminobutyric acid (1 to 100 μm) and were blocked by bicuculline, a GABA receptor blocker . On the other hand, Substance P inhibited 30.085% of the GABA activation current. Conclusions Since substance P receptor and γ - aminobutyric acid receptor coexist in the membrane of Drosophila melanogaster DGR neurons and the activation of substance P receptor modulates γ - aminobutyric acid receptor activation, the release of P Substance can reverse the presynaptic response mediated by γ-aminobutyric acid