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目的：测定血浆中磺胺二甲嘧啶及其代谢物，研究其在体内乙酰化表型。方法：用乙腈直接沉淀血浆蛋白，处理后样品在流动相为乙腈水醋酸（１６．５∶８３．５∶０．０５），流速１．０ｍｌ·ｍｉｎ－１，紫外测定波长２６０ｎｍ条件下进样。结果：磺胺二甲嘧啶和乙酰磺胺二甲嘧啶相关性分别为０．９９９９和０．９９９６。方法的平均回收率为（９９．４９±３．６２）％和（９９．８９±０．８７）％。１２０名健康志愿者服用磺胺二甲嘧啶片后，用本方法测定体内药物浓度，得快乙酰化者与慢乙酰化者之比为１０１∶１９。结论：方法简单、快速、灵敏，能较好地Ｎ乙酰化酶表型。
Objective: To determine sulfamethazine and its metabolites in plasma and study its acetylation phenotype in vivo. Methods: The plasma protein was directly precipitated with acetonitrile. After the sample was treated with acetonitrile-water-acetic acid (16.5: 83.5:0.05), the flow rate was 1.0 ml · min-1 and the UV wavelength was 260 nm Injection. Results: The correlation between sulfamethazine and acesulfame-pyrimidine was 0.9999 and 0.9996, respectively. The average recoveries of the method were (99.49 ± 3.62)% and (99.89 ± 0.87)%. 120 healthy volunteers taking sulfamethazine tablets, the determination of in vivo drug concentration by this method, the ratio of fast acetylator and slow acetylator 101:19. Conclusion: The method is simple, rapid, sensitive, and can better N acetylase phenotype.