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目的通过模拟煤矿井下被困人员断食后大鼠动物模型,动态性分析体重、脑重、脑含水量及衰竭死亡的相关性,为研究断食对脑功能影响及干预提供基础数据。方法选用华北理工大学动物实验中心提供的清洁级成年雄性12周龄健康SD大鼠36只,体重(350±30)g,随机分为正常饮食对照(A组,n=4)和只供水,不供食实验组(B组,n=32)。B组又依时序性3d、5d、7d、9d分为4个亚组(n=8),均单独分笼喂养。观测内容包括精神状态、日常行为、皮毛光泽等变化的一般行为、体重、脑重量、脑含水量。结果3d组未见一般行为有变化,5d组开始消瘦、皮下脂肪减少;7d组开始出现行走不稳;9d组开始出现死亡或处于频死状态。体重下降率随着饥饿期的延长逐渐增大,而脑重量的变化为断食7d前脑重量与正常脑重量相比较,其差异无统计学意义,只有到9d组才显示出明显的差异(P<0.05)。脑组织含水量的变化为与正常对照组相比,断食(7d、9d)组均下降(P<0.01)。结论全饥饿状态下体重随时间延长而持续下降,但在未陷入频死前脑重量均未见有统计学意义的改变,只有在9d组脑重量开始下降时,实验大鼠才会陷入频死状态或很快死亡。5d时脑含水量开始升高,大约持续1~2d后开始下降,由此推测饥饿性脑水肿或脑肿胀则可能是记忆、行为功能受损的基本原因。
OBJECTIVE: To provide basic data for the study of the effects of freshening on brain function and intervention by simulating the animal models of the rats in the underground after fasting, and dynamically analyzing the correlation between body weight, brain weight, brain water content and mortality. Methods Thirty - six healthy adult 12 - week - old male Sprague - Dawley rats were randomly divided into normal diet control (group A, n = 4), water only, Not fed the experimental group (B group, n = 32). In group B, they were divided into 4 subgroups (n = 8) according to the time sequence of 3d, 5d, 7d and 9d. Observations include general behavior such as mental status, routine behavior, changes in fur gloss, weight, brain weight, brain water content. Results There was no change in general behavior in 3d group. The weight loss began to occur in 5d group, and the subcutaneous fat decreased. The walking instability began to appear in group 7d. The onset or death occurred in group 9d. Weight loss rate gradually increased with the prolongation of the hunger period, while the change of brain weight was no significant difference between the weight of the forebrain on the 7th day after eating and the normal brain weight, and only showed significant difference to the 9th day (P <0.05). The changes of water content in brain tissue were significantly lower than those in the normal control group (P <0.01) on the 7th and 9th days. Conclusion The body weight of rats in all starvation continued to decrease with time, but there was no significant change in the frequency of forebrain weight loss. Only when the brain weight of rats in group 9d began to decrease, did rats die in frequency Status or soon die. 5d brain water content began to rise, about 1 ~ 2d continued to decline, suggesting that hunger brain edema or brain swelling may be the basic memory, behavioral impairment.