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原位ＰＣＲ技术原位ＰＣＲ即是将ＰＣＲ技术的高效扩增特点与原位杂交的细胞定位方法相结合，在组织细胞原位检测单拷贝或低拷贝的特定ＤＮＡ或ＲＮＡ序列．首先将待检标本经化学固定，以保持组织细胞良好的形态结构。经蛋白酶处理增加细胞膜及核膜通透性，使ＰＣＲ扩增反...
In-situ PCR In-situ PCR combines the efficient amplification of PCR with the in situ hybridization of cell-based methods to detect single or low copy specific DNA or RNA sequences in situ in tissue cells. First, the specimens to be seized chemically fixed to maintain a good morphological structure of the cells. After protease treatment to increase cell membrane and nuclear membrane permeability, so that PCR amplification ...