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目的研究青岛地区婴幼儿腹泻患者星状病毒流行病学特点和基因型别。方法收集青岛地区散发儿童病毒性腹泻患者粪便标本48份,经胶体金法、RT-PCR法分别检测轮状病毒和诺如病毒后,应用RT-PCR进行星状病毒检测,并对阳性标本进行测序,鉴定型别。结果 48份粪便标本中,4份检出星状病毒(8.3%),经序列分析鉴定为HastV-1型。结论青岛地区散发病毒性腹泻患儿存在星状病毒感染,型别为HastV-1。
Objective To study the epidemiological characteristics and genotypes of astrovirus in infants with diarrhea in Qingdao. Methods 48 samples of children with viral diarrhea were collected from Qingdao. Samples of rotavirus and Norovirus were detected by colloidal gold method and RT-PCR respectively. RT-PCR was used to detect the astrovirus and positive samples Sequencing, identification of type. Results Of the 48 stool specimens, 4 (8.3%) were detected and identified as HastV-1 by sequence analysis. Conclusion There are astroviral infections in children with viral diarrhea in Qingdao. The type is HastV-1.