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目的观察不同剂量和不同时间三甲基氯化锡(TMT)对小鼠小肠推进功能的影响。方法将32只小鼠按体重随机分为4组,3个染毒组剂量分别为1.00、2.15和4.64 mg/kg染毒,对照组给予生理盐水,均在染毒后24 h进行肠道推进功能指标的测定;另外,再将36只小鼠按体重随机分为6组,3个TMT染毒组以3.16 mg/kg腹腔注射染毒后在1、3和6天分别进行肠道推进功能指标的测定,3个对照组给予等量生理盐水,处理方法同对照组。结果 1.00和2.15 mg/kg染毒后24 h,肠道推进距离和推进率无明显改变;4.64 mg/kg染毒后24 h肠道推进距离为(26.71±10.59)cm,推进率为(40.12±16.13)%,较对照组(36.50±6.68)cm和(58.70±12.68)%均明显降低(P<0.05或P<0.01),3.16 mg/kg染毒后1和6天肠道推进功能无改变,在染毒后第3天推进距离为(31.00±2.45)cm,推进率为(47.54±4.14)%,较对照组(38.33±6.77)cm和(62.53±12.02)%有明显降低(P<0.05)。结论 TMT低剂量染毒对肠道推进功能作用不明显,高剂量产生抑制,3.16 mg/kg染毒后3天对肠道推进功能有抑制作用,6天后肠道推进功能逐渐恢复。
Objective To observe the effect of different doses and different time of trimethyltin chloride (TMT) on intestinal propelling function in mice. Methods Thirty-two mice were randomly divided into four groups according to body weight. The doses of the three exposure groups were 1.00, 2.15 and 4.64 mg / kg, respectively. The control group was given normal saline, and the intestinal tract was propelled 24 h after exposure. In addition, 36 mice were randomly divided into 6 groups according to body weight. Three TMT groups were given intragastric injection of 3.16 mg / kg and were respectively given intestinal propulsion at 1, 3 and 6 days The indexes were measured, the three control groups were given the same amount of saline, the treatment method was the same as the control group. Results The intestinal propulsion distance and propulsion rate did not change significantly at 24 h after 1.00 and 2.15 mg / kg exposure. The intestinal propulsion distance at 24 h after exposure to 4.64 mg / kg was (26.71 ± 10.59) cm and the propulsion rate was (40.12 ± 16.13)%, which were significantly lower than those in the control group (36.50 ± 6.68) cm and (58.70 ± 12.68)%, respectively (P <0.05 or P <0.01) (31.00 ± 2.45) cm on the third postnatal day, and the advancing rate was (47.54 ± 4.14)%, which was significantly lower than that of the control group (38.33 ± 6.77) cm and (62.53 ± 12.02)% <0.05). Conclusion The low dose of TMT has no obvious effect on the function of intestinal tract propulsion, but inhibited at high dose. The intestinal propulsion function was inhibited 3 days after exposure to 3.16 mg / kg, and the function of intestinal propulsion recovered gradually after 6 days.