论文部分内容阅读
[Objective]To investigate the extraction technology of saponins from lily(Lilium brownii) and the anti-depressant activity of saponins. [Methods]The saponins extraction was optimized by an L9(34) orthogonal design with the volume fraction of ethanol,amount of ethanol,extraction time as factors,using saponins extraction rate as index. Kunming mice tested were divided into normal control group(constant volume of normal saline),fluoxetine group(0. 004 g /kg) and high-dose,medium-dose and low-dose lily saponins groups(0. 1,0. 05 and0. 025 g /kg). The duration of immobility within 4 min were recorded in tail suspension test and forced swimming test. Body temperatures of mice were recorded at 4 h after modeling in reserpine-induced hypothermia test. [Results]The optimal extraction technology of lily saponins was as follows: extraction with 70% ethanol once,lasting for 3 h. Compared with normal control group,the duration of immobility within 4 min in tail suspension test and forced swimming test were decreased in medium-dose and low-dose lily saponins groups(P < 0. 05); the body temperature of mice was decreased at 4 h after modeling(P < 0. 05). [Conclusions]Lily saponins have certain anti-depressant activity in mice.
[Objective] To investigate the extraction technology of saponins from lily (Lilium brownii) and the anti-depressant activity of saponins. [Methods] The saponins extraction was optimized by an L9 (34) orthogonal design with the volume fraction of ethanol, amount of ethanol, extraction time as factors, using saponins extraction rate as index. Kunming mice tested were divided into normal control group (constant volume of normal saline), fluoxetine group (0.004 g / kg) and high-dose, medium-dose and The duration of immobility within 4 min were recorded in tail suspension test and forced swimming test. Body temperatures of mice were recorded at 4 h after modeling in reserpine-induced hypothermia test. [Results] The optimal extraction technology of lily saponins was as follows: extraction with 70% ethanol once, lasting for 3 h. Compared with normal control group, the duration of immobility within 4 min in tail suspension test and forced swimming t The body temperature of mice was decreased at 4 h after modeling (P <0.05). [Conclusions] Lily saponins have certain anti -depressant activity in mice.