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目的采用高效液相色谱法测定甲磺酸帕珠沙星的药物代谢动力学。方法样品经50%三氟醋酸直接沉淀蛋白后,用Hypersil BDS柱分离,以氧氟沙星为内标,采用紫外检测。结果日内、日间误差分别<6.5%和<15%,平均回收率为58.05%±4.26%。在47~24 080 ng.mL-1血浆浓度范围内呈线性关系(r2=0.997)。最低检测浓度为25 ng.mL-1。结论此法操作简便、快速。应用此法研究了30名健康志愿者30 min静脉滴注不同剂量注射用甲磺酸帕珠沙星试验制剂(0.5、1.0、1.5 g加入100 mL 0.9%氯化钠溶液中)后的药物动力学。
Objective To determine the pharmacokinetics of pazufloxacin mesylate by high performance liquid chromatography. Methods The samples were directly precipitated with 50% trifluoroacetic acid, separated by Hypersil BDS column, ofloxacin as internal standard, and detected by UV. The results of intraday and interday errors were <6.5% and <15%, the average recovery rate was 58.05% ± 4.26%. There was a linear relationship (r2 = 0.997) between 47 and 2480 ng · mL-1 plasma concentrations. The minimum detectable concentration is 25 ng.mL-1. Conclusion This method is simple and fast. This method was used to study the pharmacokinetics of 30 healthy volunteers after intravenous infusion of pazufloxacin mesylate (0.5, 1.0, 1.5 g in 100 mL of 0.9% sodium chloride solution) at different doses for 30 min learn.