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目的研究腺病毒介导水通道基因对小型猪腮腺放射损伤的治疗效果及其相应表达关系。方法19只实验用小型猪一侧腮腺给予20Gy放射剂量照射,照射后17周,转导不同浓度的水通道蛋白基因、荧光素酶基因和病毒缓冲液,分析唾液流率变化和目的基因表达。结果109pfu AdCMVhAQP1转导后3天和7天腮腺唾液流率明显增加,分别恢复到放射前腮腺唾液流率81±18%(P=0.024)和69±20%(P=0.058),14天下降到转导前水平,对照组均未见腮腺唾液流率明显变化。转导109pfu AdCMVhAQP1组免疫组织化学显示,AQP1主要表达在导管上皮,Western blotting分析有外源性水通道蛋白表达,而对照组未见AQP1表达。结论109pfu AdCMVhAQP1明显增加小型猪腮腺放射损伤唾液流率,转导的水通道基因具有潜在治疗涎腺放射损伤的临床应用价值。
Objective To investigate the therapeutic effect of adenovirus-mediated aquaporin gene on radiation-induced parotid gland injury in miniature pigs and its corresponding expression. Methods Twenty-nine mice were irradiated with 20 Gy of parotid gland on the parotid gland of piglets. After 17 weeks of irradiation, different concentrations of aquaporin gene, luciferase gene and virus buffer were transduced. The saliva flow rate and gene expression were analyzed. Results The salivary flow rate of parotid glands in 109pfu AdCMVhAQP1 was significantly increased at 3 and 7 days after transduction, and the salivary flow rate of the parotid gland was 81 ± 18% (P = 0.024) and 69 ± 20% (P = 0.058) To the level of pre-transduction, the control group showed no significant changes in the parotid salivary flow rate. Transduction of 109pfu AdCMVhAQP1 group immunohistochemistry showed that AQP1 mainly expressed in the ductal epithelium, Western blotting analysis of extrinsic aquaporin expression, but no AQP1 expression in the control group. Conclusion 109pfu AdCMVhAQP1 can significantly increase salivary flow rate in parotid glands of miniature pigs. The transduced water channel genes have potential value in the treatment of salivary gland radiation injury.