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为明确DHN黑色素合成抑制剂三环唑对玉米大斑病菌Setosphaeria turcica致病力的影响,通过含毒介质系统研究了三环唑作用下玉米大斑病菌的黑色素生物合成量、附着胞的细胞孔径、膨压和病菌的致病力。结果显示,当三环唑浓度≤5μg/mL时,玉米大斑病菌菌落生长量和产孢量变化不显著,但黑色素的生成量降至对照菌株的43%;三环唑处理的病菌附着胞细胞壁孔径增大至2.7～3.3 nm,膨压降至3.75 MPa,侵染率下降37.5%,同时病斑面积显著减小。表明三环唑通过抑制玉米大斑病菌DHN黑色素合成,降低了病菌附着胞的膨压,从而导致致病力下降。
In order to clarify the effect of tricyclazole, a DHN melanin inhibitor, on the pathogenicity of Setosphaeria turcica, the melanin biosynthesis, the cell attachment pore size , Turgor and pathogenic bacteria. The results showed that when the concentration of tricyclazole was less than or equal to 5μg / mL, the growth and sporulation of S. turcica did not change significantly, but the production of melanin decreased to 43% of that of the control strain. Tricyclazole- Cell wall diameter increased to 2.7 ~ 3.3 nm, turgor pressure decreased to 3.75 MPa, infection rate decreased 37.5%, while the lesion area was significantly reduced. The results showed that tricyclazole reduced the pathogeny by reducing the melanin synthesis of DHN and reducing the turgor pressure of attaching bacteria.