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目的 :研究肝癌细胞表面 Fas/ Fas L系统的变化及对其逃避机体免疫监控的影响。 方法 :采用流式细胞术和RT- PCR检测肝癌细胞株 Hep G2、Hep3B和 PL C/ PRF/ 5 Fas和 Fas L的表达 ;观察化疗药物争光霉素诱导肝癌细胞 Fas L表达及 Fas L表达上调后的肝癌细胞触发淋巴细胞株 Jurkat细胞凋亡的作用。结果 :Hep G2、Hep3B和 PL C/ PRF/ 5肝癌细胞株Fas表达均阴性 ;Hep G2 Fas L m RNA的表达为阴性 ,而 Hep3B和 PL C/ PRF/ 5 Fas L m RNA的表达均阳性。经争光霉素(0 .6 mg/ m l)诱导后 ,上述肝癌细胞 Fas的表达无明显变化 ,而其 Fas L的表达却增加 ,与 Jurkat细胞混合孵育则触发后者发生显著性凋亡。结论 :肝癌细胞可能一方面降低或不表达 Fas而对 Fas介导的凋亡耐受 ,另一方面又增强自身 Fas L的表达以诱发与之接触的 T淋巴细胞凋亡 ,在完成免疫逃避的同时 ,可对周围的 T细胞实施反攻击
Objective: To study the changes of Fas / Fas L system on liver cancer cells and the effect of evasion on immune surveillance. Methods: The expressions of Hep G2, Hep3B and PL C/PRF/5 Fas and Fas L were detected by flow cytometry and RT-PCR. The Fas L expression and up-regulation of Fas L expression in hepatoma cells induced by bleomycin-induced bleomycin were observed. After the liver cancer cells trigger the apoptosis of lymphocyte strain Jurkat cells. Results: Fas expression was negative in Hep G2, Hep3B and PL C/PRF/5 hepatoma cell lines; Hep G2 Fas L m RNA was negative, while Hep3B and PL C/PRF/5 Fas L m RNA were positive. After induction with bleomycin (0.6 mg/ml), there was no significant change in the expression of Fas in these hepatoma cells, but the expression of Fas L was increased, and mixed incubation with Jurkat cells triggered significant apoptosis in the latter. Conclusion: Hepatoma cells may have Fas-mediated apoptosis resistance on the one hand, but not on Fas, or on the other hand, enhance the expression of Fas L on their own to induce apoptosis of T-lymphocytes with which they are exposed. At the same time, anti-attack against surrounding T cells