论文部分内容阅读
目的研究姜黄素对孤独症鼠行为及脑源性神经营养因子(BDNF)表达的影响。方法参照Schneider的方法在大鼠孕12.5 d时模型组母鼠腹腔注射丙戊酸钠(VPA)600 mg.kg-1建立孤独症动物模型,对仔鼠进行发育学、行为学、形态学鉴定。随机选取35 d孤独症鼠20只、盐水(NS)组鼠10只。NS组鼠为出正常对照组,孤独症鼠随机分为孤独症溶剂对照(VC)组10只、孤独症姜黄素干预(VPA-C)组10只。VPA-C组鼠于出生后35 d连续2周腹腔注射姜黄素50 mg.kg-1[姜黄素用含1 mL.L-1二甲基亚砜(DMSO)的PBS液配成10 g.L-1的溶液]。NS组、VC组鼠于生后35 d连续2周每日腹腔注射等量的含1 mL.L-1DMSO的PBS液。观察姜黄素对孤独症鼠社交行为及重复理毛行为的影响,应用免疫组织化学染色法观察姜黄素干预后BDNF的表达情况。应用SPSS 18.0软件进行统计学处理。结果姜黄素干预后,孤独症鼠社交行为较干预前次数增加(P<0.05),社交行为潜伏期缩短(P<0.001),重复理毛行为累积时间减少(P<0.01);姜黄素干预后孤独症鼠大脑颞叶皮质BDNF阳性细胞积分光密度(IOD)值较干预前增加(P<0.05)。结论姜黄素对孤独症有一定的治疗作用,可一定程度改善孤独症鼠异常行为,其机制可能通过增加BDNF表达发挥作用。
Objective To investigate the effect of curcumin on the behavior and the expression of brain-derived neurotrophic factor (BDNF) in autistic rats. Methods Schneider’s method was used to establish an autistic animal model by intraperitoneal injection of sodium valproate (VPA) 600 mg.kg-1 into the model group at 12.5 d of gestation. The pups were subjected to developmental, behavioral and morphological evaluation . Twenty mice were randomly selected from 35 d autotrophs and 10 rats from saline (NS) group. Rats in NS group were normal control group. Autism mice were randomly divided into 10 autistic control group (VC) and 10 autistic curcumin intervention group (VPA-C). VPA-C mice were intraperitoneally injected with curcumin 50 mg.kg-1 for 35 d after birth for 2 weeks. Curcumin was intragastrically administered with 10 mL of PBS solution containing 1 mL of dimethylsulfoxide (DMSO) 1 solution]. The rats in NS group and VC group were injected intraperitoneally with equal volume of PBS solution containing 1 mL.L-1DMSO for 35 days after birth for 2 weeks. To observe the effect of curcumin on the social behavior and repeated grooming behavior of autistic mice. The expression of BDNF after curcumin intervention was observed by immunohistochemical staining. SPSS 18.0 software was used for statistical analysis. Results After curcumin intervention, the social behavior of autistic rats increased (P <0.05), the latent period of social behavior shortened (P <0.001), the time of repeated grooming decreased (P <0.01), and the effects of curcumin alone The integrated optical density (IOD) value of BDNF positive cells in the cerebral cortex of affected mice increased more than that before intervention (P <0.05). Conclusion Curcumin has a certain therapeutic effect on autism and may improve the abnormal behavior of autistic rats to a certain extent. The mechanism may be through increasing the expression of BDNF.