论文部分内容阅读
目的:观察亚硒酸钠对糖尿病小鼠肝功能、肝抗氧化功能的影响及大蒜素的防护作用。方法:采用四氧嘧啶复制小鼠糖尿病模型,检测正常组、造模组、亚硒酸钠组、大蒜素组的肝功能和肝脏抗氧化功能的指标。结果:空腹血糖大于200mg/dl的糖尿病小鼠3周内肝脏谷丙转氮酶(GPT)、谷草转氨酶(GOT)活性无明显改变,肝糖元含量有所下降,肝线粒体脂质过氧化产物MDA含量明显升高,超氧化物歧化酶(SOD)活性明显降低,但对肝微粒体及胞浆无明显影响,对肝一氧化氮(NO)含量也无明显改变。经口给予80μg/kg亚硒酸钠,可使糖尿病小鼠GPT、GOT活性明显下降,肝微粒体及胞浆MDA含量明显升高,SOD活性明显下降,肝脏NO含量极显著升高。大蒜素可明显的逆转上述变化。结论:亚硒酸钠具有明显肝毒性,大蒜素对亚硒酸钠所致肝损害有防护作用。
Objective: To observe the effect of sodium selenite on liver function and liver anti-oxidant function in diabetic mice and the protective effect of allicin. Methods: Alloxan was used to replicate mouse model of diabetes to detect the indexes of liver function and liver anti-oxidant function in normal group, model group, sodium selenite group and allicin group. Results: There was no significant change in hepatic GPT and GOT activity in diabetic mice with fasting blood glucose> 200mg / dl within 3 weeks, hepatic glycogen content decreased, and liver mitochondrial lipid peroxidation product MDA content increased significantly, superoxide dismutase (SOD) activity decreased significantly, but the liver microsome and cytoplasm had no significant effect on the liver of nitric oxide (NO) content did not change significantly. Oral administration of 80μg / kg sodium selenite could significantly decrease the activity of GPT and GOT, the content of MDA in liver microsome and cytoplasm, the activity of SOD and the content of NO in liver significantly increased. Allicin can significantly reverse the above changes. Conclusion: Sodium selenite has obvious hepatotoxicity. Allicin has a protective effect on hepatic injury induced by sodium selenite.