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目的 :探讨重组人IFN -γ(rhIFN -γ)在体外对SW1116抗原SC3A表达的影响。方法 :将rhIFN -γ与SW1116在37℃共育24、48和72h后 ,分别用免疫细胞化学染色法和流式细胞仪测定其SC3A抗原 (SC3AAg)的表达。结果:SW1116与20u/mlrhIFN -γ共育24、48和72h ,其SC3AAg 阳性细胞分别为95.5 %、93.2 %和95.2 %,PBS组分别为11.3 %、12.2 %和10.0 % ,AGZY -83 -a细胞系均未见SC3AAg 阳性细胞 ,阳性对照组的C1184细胞系SC3AAg阳性细胞80.5 %。结论 :2u/mlrhIFN -γ能使SW1116细胞的SC3AAg表达明显上调 ,其中20u/ml、24h是表达峰值的最佳浓度和时间。
Objective: To investigate the effect of recombinant human IFN-γ (rhIFN-γ) on the expression of SW1116 antigen SC3A in vitro. METHODS: After 24 h, 48 h and 72 h of incubation with rhIFN-γ and SW1116 at 37°C, the expression of SC3A antigen (SC3AAg) was determined by immunocytochemistry and flow cytometry, respectively. RESULTS: After SW1116 and 20u/ml rhIFN-γ were co-cultured for 24, 48 and 72 hours, the SC3AAg positive cells were 95.5 %, 93.2 % and 95.2 %, respectively, in the PBS group were 11.3 %, 12.2 % and 10.0 % respectively. AGZY -83 -a There were no SC3AAg positive cells in the cell lines. The positive control group of C1184 cell line was 80.5 % of SC3AAg positive cells. Conclusion : 2u/mlrhIFN-γ can significantly up-regulate the expression of SC3AAg in SW1116 cells, and 20u/ml and 24h are the optimal concentration and time for peak expression.