【摘 要】
：
Accumulating evidence indicates that mitochondrial dysfunction and oxidative stress play a pivotal role in the initiation and progression of nonalcoholic fatty liver disease(NAFLD).In this study,we found that blueberry-derived exosomes-like nanoparticles(
【机 构】
：
Chongqing Key Lab of Medicinal Chemistry&Molecular Pharmacology,Chongqing University of Technology,C
论文部分内容阅读
Accumulating evidence indicates that mitochondrial dysfunction and oxidative stress play a pivotal role in the initiation and progression of nonalcoholic fatty liver disease(NAFLD).In this study,we found that blueberry-derived exosomes-like nanoparticles(BELNs)could ameliorate oxidative stress in rotenone-induced HepG2 cells and high-fat diet(HFD)-fed C57BL/6 mice.Preincubation with BELNs decreased the level of reactive oxygen species(ROS),increased the mitochondrial membrane potential,and prevented cell apoptosis by inducing the expression of Bcl-2 and heme oxygenase-1(HO-1)and decreasing the content of Bax in rotenone-treated HepG2 cells.We also found that preincubation with BELNs accelerated the translocation of Nrf2,an important transcription factor of antioxidative proteins,from the cytoplasm to the nucleus in rotenone-treated HepG2 cells.Moreover,administration of BELNs improved insulin resistance,ameliorated the dysfunction of hepatocytes,and regulated the expression of detoxifying/antioxidant genes by affecting the distribution of Nrf2 in the cytoplasm and nucleus of hepatocytes of HFD-fed mice.Furthermore,BELNs supplementation prevented the formation of vacuoles and attenuated the accumulation of lipid droplets by inhibiting the expression of fatty acid synthase(FAS)and acetyl-CoA carboxylase 1(ACC1),the two key transcription factors for de novo lipogenesis in the liver of HFD-fed mice.These findings suggested that BELNs can be used for the treatment of NAFLD because of their antioxidative activity.
其他文献
Stratifin(SFN)is a member of the 14-3-3 family of highly conserved soluble acidic proteins,which regulates a variety of cellular activities such as cell cycle,cell growth and development,cell survival and death,and gene transcription.Acute kidney injury(A
目的 分析2015-2019年北京佑安医院手足口病住院患者病原检测与临床指标间的关联.为提高手足口病不同病原感染的鉴别诊断和预后风险提供一定的科学依据.方法 采用描述性流行病学方法分析2015-2019年北京佑安医院手足口病住院患者的一般情况、临床表现、实验室病原检测及临床指标.结果 2015-2019年北京佑安医院因手足口病住院的患者共计353例,肠道病毒核酸检测阳性的253例(71.67％),分为3组:肠道病毒A组71型(enterovirus A71,EV-A71)阳性50例(19.76％),柯萨奇
Tyrosine-decahydrofluorene derivatives feature a fused[6.5.6]tricarbocyclic core and a 13-membered para-cyclophane ether.Herein,we identified new xenoacremones A,B,and C(1-3)from the fungal strain Xenoacremonium sinensis ML-31 and elucidated their biosynt
Heart aging is characterized by structural and diastolic dysfunction of the heart.However,there is still no effective drug to prevent and treat the abnormal changes in cardiac function caused by aging.Here,we present the preventive effects of emodin and i
Panax notoginseng,a traditional Chinese medicine,exerts beneficial effect on diabetic kidney disease(DKD),but its mechanism is not well clarified.In this study we investigated the effects of ginsenoside Rb1(Rb1),the main active ingredients of Panax notogi
Acute lung injury(ALI)and its severe form acute respiratory distress syndrome(ARDS)are known as the common causes of respiratory failure in critically ill patients.Myeloid differentiation 2(MD2),a co-receptor of toll like receptor 4(TLR4),plays an importa
目的:观察荭草苷通过Notch通路对心律失常大鼠的改善作用及对心电图(ECG)的影响.方法:采用舌下注射乌头碱方式建立心律失常大鼠模型,随机分为模型组、荭草苷低剂量组(0.75 mg· kg-1)、荭草苷中剂量组(1.5 mg·kg-1)、荭草苷高剂量组(3 mg·kg-1),另设对照组.干预后监测ECG,比较心率(HR)、Q-T间期、P-R间期、QRS波群时间及室性早搏(PVC)、室性心动过速(VT)、心室颤动(VF)发生时间;HE染色观察心肌组织病理学变化;TUNEL染色观察心肌细胞凋亡情况;比较心肌
Emerging evidence shows that chronic inflammation mediated by toll-like receptors(TLRs)contributes to diabetic nephropathy.Myeloid differentiation primary-response protein-88(MyD88)is an essential adapter protein of all TLRs except TLR3 in innate immunity
Vascular calcification(VC)is characterized by pathological depositions of calcium and phosphate in the arteries and veins via an active cell-regulated process,in which vascular smooth muscle cells(VSMCs)transform into osteoblast/chondrocyte-like cells as
目的:建立以土大黄苷为标志性成分的保健食品中非法添加伪品大黄及其提取物的UPLC-QTRAP-MS/MS的分析方法.方法:样品用甲醇经超声提取,经Agilent InfinityLab Poroshell 120 SB-Cl8色谱柱(100mm×2.1 mm,2.7μm)分离,以5 mmol·L-1甲酸铵水溶液和乙腈作为流动相,进行梯度洗脱,在负离子扫描模式下,采用MRM-IDA-EPI模式检测.结果:土大黄苷质量浓度在5～100 ng·mL-1范围内线性均良好.相关系数(r2)为0.9987.土大黄苷在