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目的：研究雌激素促进人的类成骨细胞株ＴＥ８５细胞骨形成的作用。方法：用３Ｈ胸腺嘧啶、３Ｈ脯氨酸参入法分别测定细胞的增殖和胶原合成；紫外分光光度法测定细胞内骨碱性磷酸酶（ＡＬＰ）活性；放免法测定细胞内骨钙素（ＢＧＰ）含量；放射配基法测定细胞核雌激素受体结合。结果：雌激素（Ｅ２，００１～１０ｎｍｏｌ·Ｌ－１）可浓度依赖地刺激ＴＥ８５细胞的３Ｈ胸腺嘧啶和３Ｈ脯氨酸的参入量；在相同的时间点和剂量下，可增加成骨细胞内ＡＬＰ活性和ＢＧＰ的含量。结论：Ｅ２通过增加成骨细胞数量、促进细胞胶原蛋白及ＡＬＰ和ＢＧＰ的合成而促进成骨作用，这些作用是通过雌激素受体介导的。
Objective: To study the effect of estrogen on the bone formation of human osteoblast-like cell line TE85. Methods: Proliferation and collagen synthesis were measured by 3H-thymidine incorporation and 3H-proline incorporation, respectively. The activity of intracellular alkaline phosphatase (ALP) was measured by UV spectrophotometry. The levels of intracellular osteocalcin BGP) content; radioligand determination of nuclear estrogen receptor binding. Results: Estrogen (E2,0.01-10 nmol·L-1) could stimulate the 3H-thymidine and 3H-proline incorporation in TE85 cells in a dose-dependent manner. At the same time and dose, Can increase the ALP activity in osteoblasts and BGP content. Conclusion: E2 promotes osteogenesis by increasing the number of osteoblasts, promoting the synthesis of cellular collagen, ALP and BGP. These effects are mediated through the estrogen receptor.