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用循环伏安法和同步荧光光谱技术研究了肌红蛋白的电化学行为，实验结果表明，高铁肌红蛋白分子中至少存在一个可调节分子构象变化的氧分子，而且长时间通入高纯氮气可以除掉高铁肌红蛋白分子内的这个氧，当高铁肌红蛋白分子内的氧被彻底除去后，用循环伏安法可以观察到肌红蛋白在三氧化二铟电极上的准可逆的电学反应。同步荧光光谱实验表明，高铁肌红蛋白在彻底除氧后，分子构象发生了变化，而且这种构象变化是可逆的。
The electrochemical behavior of myoglobin was studied by cyclic voltammetry and synchronous fluorescence spectroscopy. The experimental results show that there is at least one oxygen molecule in the metmyoglobin molecule that can adjust the molecular conformational change, and the long-term access to high purity nitrogen The oxygen in the methotrexate can be removed and the quasi-reversible electromyography of myoglobin at the indium-oxide electrode can be observed by cyclic voltammetry after the oxygen in the methotrexate is completely removed reaction. Simultaneous fluorescence spectroscopy experiments showed that the methotrexate of metmyoglobin changed completely after oxygen was removed, and the conformational change was reversible.