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以高粱、杂交高粱、甜高粱、苏丹草、黑高粱和假高粱6种不同高粱属植物为实验材料,利用ISSR分子标记分析其遗传关系。结果表明,这6种高粱属植物在DNA水平上具有较高的遗传多样性。从110条ISSR引物中成功筛选到22条多态性高、稳定性好的引物,共扩增出182条带,其中差异性条带153条,多态条带比率(PPB)为84.0%。基于遗传距离系数的Neighbor-Joining聚类分析可以将6种高粱分为两大组,相互之间的遗传距离为0.25。另外可以根据一些多态性高的引物所扩增得到的品种特异性条带区分所有不同高粱种,如引物IR89、IS16等。这些为研究高粱属植物的分类、鉴定和进化提供了分子生物学方面的理论依据。
Sorghum, hybrid sorghum, sweet sorghum, sudangrass, black sorghum and fake sorghum six different sorghum plants as experimental materials, the use of ISSR molecular markers to analyze the genetic relationship. The results showed that these six species of Sorghum had higher genetic diversity at the DNA level. Totally 22 polymorphic and stable primers were screened from 110 ISSR primers. A total of 182 bands were amplified, of which 153 bands were differential and 84.0% bands were polymorphic bands. Neighbor-Joining clustering analysis based on genetic distance coefficient can divide six kinds of sorghum into two groups with a genetic distance of 0.25. In addition, some species-specific bands amplified by the primers with high polymorphism can be used to distinguish all the different sorghum species, such as primers IR89, IS16 and the like. These provide molecular biology theoretical basis for studying the classification, identification and evolution of Sorghum.