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目的 探讨反义寡脱氧核苷酸 (ASPODNS)对人肝癌多药耐药基因 (MDR)的逆转作用。 方法ASPONs作用于肝癌MDR细胞模型后 ,用MTT法检测其逆转作用 ,用逆转录PCR和流式细胞术检测其对 4种MDR基因表达的影响。 结果 ASPODNS 抑制MDR基因的表达存在时间 效应和剂量 效应关系 ,对单基因MDR细胞耐药性的逆转率为 90 2 6 %～ 10 0 % ,对多重MDR细胞耐药性逆转率为 70 0 9%～ 10 0 %。 结论 多种ASPODNs联合逆转多重MDR具有可行性并取得明显效果
Objective To investigate the reversal of multidrug resistance gene (MDR) in human hepatocellular carcinoma by antisense oligodeoxynucleotides (ASPODNS). Methods ASPONs were used to detect the MDR cell model of hepatocellular carcinoma. MTT assay was used to detect the effect of ASPONs on the MDR cell model. The effects of ASPONs on the expression of four MDR genes were detected by reverse transcription polymerase chain reaction and flow cytometry. Results ASPODNS inhibited the expression of MDR gene in a time-and dose-dependent manner. The reversal rates of drug resistance in single-gene MDR cells ranged from 90 2 6% to 100%, and the reversal rates of multidrug-resistant MDR cells were 70 0 9% ~ 100%. Conclusions The combination of multiple ASPODNs in the reversal of multiple MDR is feasible and achieves significant results