论文部分内容阅读
目的:考察五味子甲素对大鼠及小鼠小肠平滑肌功能的调节作用,为揭示五味子“收敛固涩”功效的机制及物质基础提供药理学依据。方法:取昆明种小鼠50只,按均衡随机分组法分为5组,分别为正常组,五味子甲素高、中、低剂量组(20,40,80 mg·kg-1),莨菪阳性组(20 mg·kg-1);另取昆明种小鼠60只,按均衡随机分组法分为6组,分别为正常组,模型组(ip,新斯的明),五味子甲素高、中、低剂量组(80,40,20 mg·kg-1),莨菪阳性组(20 mg·kg-1),ig给予相应药物,正常组及模型组给予等体积蒸馏水,每日1次,连续5 d,分别用炭末推进法分别观察五味子甲素对正常小鼠和新斯的明肠功能亢进模型小鼠肠推进的影响。以孵育在克氏液中大鼠离体小肠为模型,观察五味子甲素对大鼠离体肠平滑肌收缩性的影响。取SD大鼠30只,随机分为正常组,五味子甲素高、中、低剂量组(80,40,20 mg·kg-1),除正常组外,其余各组用番泻叶制备大鼠腹泻模型,造模成功后ig给予相应药物,每日1次,连续7 d,观察五味子甲素对腹泻模型大鼠肠平滑肌肌球蛋白轻链激酶(MLCK)表达的影响。结果:与正常组和新斯的明模型组比较,五味子甲素高、中剂量组均能显著降低正常小鼠和新斯的明肠功能亢进小鼠的小肠炭末推进率(P<0.05,P<0.01)。五味子甲素在10~80μmol·L-1可显著抑制大鼠离体小肠平滑肌的收缩;与番泻叶模型组比较,五味子甲素高、中、低剂量组均能显著抑制腹泻模型大鼠小肠MLCK蛋白的表达(P<0.01)。结论:五味子甲素可显著抑制小鼠在体和大鼠离体小肠运动,该作用可能与其抑制小肠收缩运动相关的肠平滑肌肌球蛋白轻链激酶表达相关。
OBJECTIVE: To investigate the regulatory effect of Schisandrin on intestinal smooth muscle function in rats and mice, and to provide a pharmacological basis for revealing the mechanism and material basis of the efficacy of Schisandra. Methods: Fifty Kunming mice were randomly divided into five groups according to the same method: normal group, Schizandrin A high, middle and low dose group (20, 40, and 80 mg · kg -1) (20 mg · kg-1). Another 60 Kunming mice were divided into 6 groups according to equalization randomization: normal group, model group (ip, neostigmine), schisandrin, (80,40,20 mg · kg-1) and the positive group (20 mg · kg-1). The rats in the normal group and the model group were given the same volume of distilled water once a day, For 5 consecutive days, the effect of Schisandra chinensis on the intestinal propulsion in normal mice and neostigmine-induced hyperthyroidism mice were observed respectively by charcoal propulsion method. The effects of Schisandra chinensis on contractility of isolated rat intestinal smooth muscle were observed by incubating the isolated rat small intestine in Krebs solution. 30 SD rats were randomly divided into normal group, Schisandra chinensis high, medium and low dose groups (80,40,20 mg · kg-1), except for the normal group, the other groups were prepared with senna Rat model of diarrhea. After the model was successfully established, the corresponding drugs were administered once a day for 7 consecutive days to observe the effect of schizandrin on the expression of myosin light chain kinase (MLCK) in the intestinal smooth muscle of diarrhea model rats. Results: Compared with normal group and neostigmine group, both high and medium doses of schizandrin could significantly reduce the small intestine charcoal propulsion rate in normal mice and Neostigma mice (P <0.05, P <0.01). Schisandrin at 10 ~ 80μmol·L-1 can significantly inhibit the contraction of isolated rat intestinal smooth muscle; Compared with the senna model group, Schisandrin A high, medium and low dose group can significantly inhibit the diarrhea model rat small intestine MLCK protein expression (P <0.01). Conclusion: Schisandrin can significantly inhibit the movement of isolated small intestine in vivo and in vivo in rats, which may be related to the inhibition of intestinal smooth muscle myosin light chain kinase expression.