Preparation of Ag/AgBr/TiO_2 as Catalyst Carriers and Its Damage to Plasmid DNA and Tetrahymena

来源 :Journal of Wuhan University of Technology(Materials Science | 被引量 : 0次 | 上传用户:sweetyjiaxin
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The composites based on the Ti O2 are potentially used in wetland pollution control. In this work, the biological effect of the Ag/Ag Br/Ti O2/Active carbon(AC) composites was studied on the plasmid DNA and Tetrahymena membrane. The atomic force micrograph(AFM) images showed that, in the presence of the composites under illumination, most p UC18 DNA molecules showed quite different topography and were opened and relaxed circle shapes. After DNA was catalyzed for 40 min, all supercoiled and circular DNA were changed into the linear DNA molecules. The gel electrophoresis experiment confirmed the results and demonstrated the dynamic process of DNA degradation. ATR-FTIR spectra revealed that amide groups and PO2-of the phospho-lipid phospho-diester on Tetrahymena surface were oxidized in the presence of the composites under illumination. An increase in the fluorescence polarization of DPH was observed, reflecting a significant decrease in membrane fluidity of Tetrahymena. The composites based on the Ti O2 are potentially used in wetland pollution control. In this work, the biological effect of the Ag / Ag Br / Ti O2 / Active carbon (AC) composites was studied on the plasmid DNA and Tetrahymena membrane. force micrograph (AFM) images showed that, in the presence of the composites under illumination, most p UC18 DNA molecule showed quite different topography and were opened and relaxed circle shapes. After DNA was catalyzed for 40 min, all supercoiled and circular DNA were changed ATR-FTIR spectra revealed that amide groups and PO2-of the phospho-lipid phospho-diester on Tetrahymena surface were oxidized in the presence of the composites under illumination. An increase in the fluorescence polarization of DPH was observed, reflecting a significant decrease in membrane fluidity of Tetrahymena.
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