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目的：研究ＥＤＲＦ（ｅｎｄｏｔｈｅｌｉｕｍ－ｄｅｒｉｖｅｄｒｅｌａｘｉｎｇｆａｃｔｏｒ，ＥＤＲＦ）对ＮＥ（ｎｏｒｅｐｉｎｅｐｈｒｉｎｅ，ＮＥ）引起的大鼠主动脉收缩反应的影响。方法：内皮完整和去内皮的大鼠主动脉环悬挂在器官浴槽中，测定血管的张力和收缩速度的变化。所有的实验在吲哚美辛（ｉｎｄｏｍｅｔｈａｃｉｎ，１０μｍｏｌ·Ｌ－１）和普萘洛尔（ｐｒｏｐｒａｎｏｌｏｌ，３μｍｏｌ·Ｌ－１）存在下进行。结果：用甲烯蓝（ｍｅｔｈｙｌｅｎｅｂｌｕｅ，ＭＢ，１０μｍｏｌ·Ｌ－１）和左旋硝基精氨酸（ＮＧ－ｎｉｔｒｏ－Ｌ－ａｒｇｉｎｉｎｅ，Ｌ－ＮＮＡ，３０μｍｏｌ·Ｌ－１）处理内皮完整的大鼠主动脉环，ＮＥ的剂量－收缩曲线明显左移。ＥＣ３０值均降低７倍，最大反应比值分别为１．６±０．３和１．７±０．４。在去内皮的大鼠主动脉环中，经ＭＢ与Ｌ－ＮＮＡ处理后，仍可见ＥＣ３０下降３倍，最大反应比值分别为１．１±０．６和１．１±０．４。后者可能与血管平滑肌产生少量ＥＤＲＦ有关。结论：结果提示，ＮＥ对血管的收缩反应也受血管内皮和平滑肌产生的ＥＤＲＦ的调控。
Objective: To investigate the effect of EDRF on norepinephrine (NE) -induced aortic contractile response in rats. METHODS: Endothelial complete and endothelium-derived rat aortic rings were suspended in organ baths and the changes in vascular tone and contractile velocity were measured. All experiments were performed in the presence of indomethacin (10 μmol·L-1) and propranolol (3 μmol·L-1). RESULTS: Endothelialized rat heads were treated with methyleneblue (MB, 10 μmol·L-1) and NG-nitro-L-arginine (L-NNA, 30 μmol·L-1) Arterial ring, NE dose-contraction curve shifted to the left significantly. EC30 values were reduced 7 times, the maximum response ratio was 1.6 ± 0.3 and 1.7 ± 0.4. In the aortic rings of endothelium-deprived rats, EC30 decreased 3-fold after treatment with MB and L-NNA, and the maximum response ratios were 1.1 ± 0.6 and 1.1 ± 0.4, respectively. The latter may be related to a small amount of EDRF produced by vascular smooth muscle. CONCLUSIONS: The results suggest that the contractile response of NE to blood vessels is also regulated by the EDRF produced by the vascular endothelium and smooth muscle.