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[目的]研究葡萄糖转运蛋白-1(GLUT-1)和β-连接蛋白(β-catenin)表达与宫颈癌发生、发展及其生物学行为的关系。[方法]应用免疫组化方法检测20例正常宫颈﹑25例宫颈上皮内瘤变(CIN)、40例宫颈癌组织中GLUT-1及β-catenin表达水平。[结果]GLUT-1在CIN组织和宫颈癌组织中均有阳性表达,在正常对照组中无表达。宫颈癌组织中GLUT-1表达率显著高于CIN组(82.5%vs.52%,P=0.006);宫颈癌Ⅱ期中GLUT-1表达率显著高于Ⅰ期(88.89%vs.69.23%,P=0.044);淋巴结转移的宫颈癌组中GLUT-1表达高于无淋巴结转移的宫颈癌组(100%vs.69.57%,P=0.014);各期宫颈癌组、各病理分级中GLUT-1表达均无统计学意义(P=0.216)。宫颈癌组织中GLUT-1阳性表达率与临床期别和淋巴结转移有关,与组织病理分级无关。β-catenin在正常对照组未发现异常表达。宫颈癌组β-catenin异常表达率显著高于CIN组(85%vs.52%,P=0.009),但不同分期宫颈癌组β-catenin异常表达率无显著性差异(77%vs.89%,P=0.370);β-catenin异常表达的宫颈癌组中GLUT-1阳性率显著高于β-catenin正常表达的宫颈癌组(91.18%vs.33.33%,P<0.001),且宫颈癌组中β-catenin异常表达和GLUT-1阳性表达成正相关(r=0.584,P<0.001)。[结论]联合检测宫颈癌组织中GLUT-1和β-catenin的表达有助于了解宫颈癌生物学行为,为进一步指导临床治疗与判断预后提供帮助。
[Objective] To investigate the relationship between the expression of GLUT-1 and β-catenin and the occurrence, development and biological behavior of cervical cancer. [Method] Immunohistochemistry was used to detect the expression of GLUT-1 and β-catenin in 20 cases of normal cervix, 25 cases of cervical intraepithelial neoplasia (CIN) and 40 cases of cervical cancer. [Results] GLUT-1 was positively expressed in both CIN and cervical cancer tissues, but not in normal controls. The positive rate of GLUT-1 in cervical cancer was significantly higher than that in CIN (82.5% vs.52%, P = 0.006). The positive rate of GLUT-1 in stage II cervical cancer was significantly higher than that in stage I (88.89% vs.69.23%, P = 0.044). The expression of GLUT-1 in cervical cancer with lymph node metastasis was higher than that in cervical cancer without lymph node metastasis (100% vs.69.57%, P = 0.014) Expression was not statistically significant (P = 0.216). The positive rate of GLUT-1 expression in cervical cancer was related to clinical stage and lymph node metastasis, but not to histopathological grade. β-catenin was not found in normal control group. The abnormal expression of β-catenin in cervical cancer group was significantly higher than that in CIN group (85% vs.52%, P = 0.009), but there was no significant difference in the expression ofβ-catenin in cervical cancer group (77% vs.89% , P = 0.370). The positive rate of GLUT-1 in cervical cancer with abnormal expression of β-catenin was significantly higher than that in cervical cancer with normal expression of β-catenin (91.18% vs.33.33%, P <0.001) There was a positive correlation between the abnormal expression of β-catenin and the positive expression of GLUT-1 (r = 0.584, P <0.001). [Conclusion] The combined detection of GLUT-1 and β-catenin expression in cervical cancer will help to understand the biological behavior of cervical cancer and provide guidance for further clinical treatment and prognosis.