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[Objective] The research was to establish the method of determining the content of chlorogenic acid in compound Shilintong capsule. [Method] The high performance liquid chromatography was adopted: chromatographic column: ZORBAXSB-C18(5 μm, 4.6 mm×250 mm), moving phase: acetonitrile-0.2% phosphoric acid solution(8∶92 V/V), flowing speed: 1.2 ml/min, detected wavelength: 327 nm. [Result] Chlorogenic acid showed good linear relationship within the range of 0.04-0.08 μg/ml. The sample recovery rate was 101.7%, RSD was 1.88%(n=6). [Conclusion] Using high performance liquid chromatography to determine the content of chlorogenic acid in compound Shilintong capsule has many advantages, such as simple operation process, accuracy, good repetitiveness, high precision and strong specificity. Therefore, it could be used as the content determination standard of compound Shilintong capsule.
[Objective] The research was to establish the method of determining the content of chlorogenic acid in compound Shilintong capsule. [Method] The high performance liquid chromatography was adopted: chromatographic column: ZORBAXSB-C18 (5 μm, 4.6 mm × 250 mm) Moving phase: acetonitrile-0.2% phosphoric acid solution (8:92 V / V), flowing speed: 1.2 ml / min, detected wavelength: 327 nm. [Result] Chlorogenic acid showed good linear relationship within the range of 0.04-0.08 μg / ml. The sample recovery rate was 101.7%, RSD was 1.88% (n = 6). [Conclusion] Using high performance liquid chromatography to determine the content of chlorogenic acid in compound Shilintong capsule has many advantages, such as simple operation process, accuracy, good repetitiveness, high precision and strong specificity. Therefore, it could be used as the content determination standard of compound Shilintong capsule.