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我们从秦岭引来一种卷丹(Lilium tigrinum),由于有较好的观赏价值,进行了组培育苗。培养温度为23—25℃,光强度1000勒克司,每天光照10小时,其具体方法如下: 7月初,将未成熟的珠芽取下,消毒后一切为二,置于愈伤组织诱导培养基(MS培养基每升需添加NAA0.1毫克和BA0.5毫克)上,10天后珠芽开始分化愈伤组织,待它成倍增长时,切开、部分转入分化培养基(在MS中每升需添
We induced a kind of Lilium tigrinum from the Qinling Mountains. Because of its good ornamental value, we conducted tissue culture seedlings. The culture temperature was 23-25 ° C, the light intensity was 1000 lux, and the light was applied for 10 hours everyday. The specific methods are as follows: In early July, the immature buds were removed and all the two were sterilized to be placed in callus induction medium MS medium supplemented with NAA0.1 mg / L and BA0.5 mg / L). Ten days later the germ began to differentiate into calluses and, when multiplied, cut and partially transferred into differentiation medium (in MS per liter Need to Tim