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本文报道了番茄叶片诱导植株再生体系和TCS基因转化体系的研究方法,“皮克”、“美引11”2个番茄品种叶片的再生植株诱导率达到70%以上;根的分化率达到90%以上;同时,筛选出的TCS基因转化体得到了分子生物学的证明,番茄品种的转化率分别为1.13%和0.72%。
In this paper, tomato leaf-induced plant regeneration system and TCS gene transformation system research methods, “Pick”, “Mei cited 11” two tomato varieties of leaf regeneration rate of more than 70%; root differentiation rate of 90% At the same time, the TCS transformants were confirmed by molecular biology. The conversion rates of tomato varieties were 1.13% and 0.72%, respectively.