论文部分内容阅读
采用聚丙烯酰胺垂直板电泳技术对蒙古冰草与航道冰草正、反交杂种F1代与其染色体加倍植株的POD同工酶酶谱表征进行了分析。结果表明:在分蘖期、抽穗期2个不同发育阶段,蒙古冰草×航道冰草正交杂种F1代染色体加倍植株、航道冰草×蒙古冰草反交杂种F1代染色体加倍植株与蒙古冰草×航道冰草正交杂种F1代植株、航道冰草×蒙古冰草反交杂种F1代植株、蒙古冰草亲本、航道冰草亲本的POD同工酶酶带在数目、位点及强弱上均存在一定差异,同工酶酶谱表征可作为正、反交杂种F1代与其染色体加倍植株在蛋白质水平相互识别的重要依据;在对不同植物材料鉴定时从几个不同的生育阶段做同工酶酶谱对比分析,要比单一生育阶段更能反映各材料间酶谱的遗传差异性,提高其鉴定结果的可靠性。
The polyacrylamide vertical plate electrophoresis was used to characterize the POD isozyme patterns of the F1 progeny and their chromosome doubling plants in the hybrids of P. alterniflora and P. aeruginosa. The results showed that in F1 tillering stage and heading stage, F1 hybrids of Agropyron chinensis × Trail Agropyron crosses F1, doubled plants of Chromosome Ⅱ, × POD isozymes in ice grass cross hybrid F1 plants, waterway ice grass × Mongolian ice grass hybrid F1 plants, Mongolia ice grass parents, waterway ice grass parents POD isozymes in the number, location and strength There are some differences, isoenzyme zymogram characterization can be used as positive and negative hybrid F1 generation and its chromosome doubling of the important basis for the identification of the protein level; in the identification of different plant materials from several different stages of fertility to do the same work Enzyme zymogram comparative analysis, than the single stage of growth can better reflect the genetic differences between the enzymes of the various materials to improve the reliability of the identification results.