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目的:对比研究外周血miR-208b与心肌肌钙蛋白T(cTnT)在早期诊断急性心肌梗死(AMI)中的价值。方法:采集67名AMI患者和32名非AMI对照者的外周血浆,通过定量逆转录聚合酶链反应(qRTPCR)测定miR-208b的浓度,通过电化学发光法检测cTnT的相应波动。结果:AMI患者外周血miR-208b的浓度较非AMI对照者明显升高(P<0.01),AMI患者痊愈出院时miR-208b的血浆浓度已回落至基线,miR-208b浓度的变化与研究病例的自身特征无明显相关性(P>0.05)。另外,受试者工作特征曲线(ROC)显示,miR-208b在早期诊断AMI方面具有高度的心肌特异性和敏感性,但并不优于cTnT。结论:外周血miR-208b可作为AMI早期诊断的标志物,但在诊断价值上不优于cInT。
Objective: To compare the value of peripheral blood miR-208b and cardiac troponin T (cTnT) in the early diagnosis of acute myocardial infarction (AMI). METHODS: Peripheral blood plasma was collected from 67 AMI patients and 32 non-AMI controls. The concentration of miR-208b was determined by quantitative reverse transcription polymerase chain reaction (qRTPCR), and the corresponding fluctuations of cTnT were detected by electrochemiluminescence. Results: The concentration of miR-208b in peripheral blood of patients with AMI was significantly higher than that of non-AMI controls (P <0.01). The plasma concentration of miR-208b in patients with AMI was recovered to baseline after AMI was discharged. The changes of miR- No significant correlation between their own characteristics (P> 0.05). In addition, the receiver operating characteristic curve (ROC) showed that miR-208b has a high degree of myocardial specificity and sensitivity in the early diagnosis of AMI but is not superior to cTnT. Conclusion: Peripheral blood miR-208b can be used as a marker for the early diagnosis of AMI, but it is not superior to cInT in diagnostic value.