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采用从人精浆中纯化的前列腺特异性抗原（ＰＳＡ），经免疫、融合、筛选，得到两株抗ＰＳＡ抗原的单克隆抗体１Ｈ７和２Ｄ１，并通过免疫组化法鉴定了抗体的特异性。将其配对建立的定量测定血清ＰＳＡ的双抗体夹心ＥＬＩＳＡ法最低检出量为０．５μｇ／Ｌ，孔间平均变异系数为４．４％，批内平均变异系数为５．８％。３５例正常男性血清ＰＳＡ的范围（ｘ±ｓ）为（１．４５±１．２４）μｇ／Ｌ；１１例前列腺癌患者血清ＰＳＡ的范围（ｘ±ｓ）为（２６±２７．２）μｇ／Ｌ。与进口ＰＳＡ－ＥＬＩＳＡ试剂盒检测的总符合率为８３％。
Purified prostate specific antigen (PSA) from human seminal plasma was used for immunization, fusion and screening to obtain two anti-PSA antigen monoclonal antibodies 1H7 and 2D1. The specificity of the antibody was identified by immunohistochemistry. The minimal detectable amount of double antibody sandwich ELISA for the quantitative determination of serum PSA was 0.5 μg/L. The average coefficient of variation between wells was 4.4%, and the average intra-assay coefficient of variation was 5.8%. The range of serum PSA (x±s) in 35 normal men was (1.45±1.24) μg/L; the range of serum PSA (x±s) in 11 patients with prostate cancer was (26±27.2) μg. /L. The total compliance with the imported PSA-ELISA kit was 83%.