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目的评价聚乙烯亚胺(polyethylenimine,PEI)多阳离子聚合体对青霉素类抗生素体外抑制铜绿假单胞菌(Pscudomonas aeruginosa,100609)活性的影响。方法采用光密度法测定PEI多阳离子聚合体分别与4种青霉素类抗生素联合使用对铜绿假单胞菌的最小抑菌浓度的影响。同时使用光密度法测定不同浓度相对分子质量为10000PEI多阳离子聚合体与亚最小抑菌浓度(11.5%MIC的浓度)的替卡西林共同作用对P.aeruginosa,100609的抑菌作用并计算抑菌百分数。结果相对分子质量为10000,260mg·L-1的PEI分别使替卡西林,羧苄西林,氨苄西林和哌拉西林的MIC降低4～64倍。在细菌动力学致死-增殖研究中,发现PEI可显著增强替卡西林对P.aeruginosa,100609的抑菌作用,且其作用与PEI的浓度存在正相关性,表明PEI与青霉素类抗生素具有协同作用。结论PEI多阳离子聚合体可以显著增强青霉素类药物对P.aeruginosa,100609的抑菌活性。
Objective To evaluate the effect of polyethylenimine (PEI) polycation on the activity of penicillin antibiotics against Pseudomonas aeruginosa (100609) in vitro. Methods The optical density method was used to determine the minimum inhibitory concentration (MIC) of Pseudomonas aeruginosa (Pseudomonas aeruginosa) in combination with 4 kinds of penicillins and PEI polycation. At the same time, the optical density method was used to determine the bacteriostasis of P.aeruginosa and 100609 with different concentrations of polycationic polyelectrolyte with a molecular weight of 10000PEI and ticarcillin with the minimum inhibitory concentration (11.5% MIC) percentage. Results PEI with relative molecular mass of 10000 and 260mg · L-1 reduced the MIC of ticarcillin, carbenicillin, ampicillin and piperacillin by 4 ~ 64 times, respectively. In the bacteriological lethal-proliferation study, we found that PEI can significantly enhance the antibacterial activity of ticarcillin against P.aeruginosa 100609 and its effect is positively correlated with the concentration of PEI, indicating that PEI has synergistic effect with penicillin antibiotics . Conclusion PEI polycationic polymer can significantly enhance the antibacterial activity of penicillin against P. aeruginosa 100609.