论文部分内容阅读
目的采用多肽固相合成法合成蓝贻贝抗凝肽MEAP(Blue mussel Mytilus edulis anticoagulant peptide),初步研究其抗凝活性。方法以2-CTC树脂为载体,Fmoc保护氨基酸为原料,按照蓝贻贝抗凝肽的序列从C端向N端逐步偶联氨基酸,裂解后得到粗肽,经半制备液相纯化后测定精肽的凝血酶时间(TT)、活化的部分凝血活酶时间(APTT)、凝血酶原时间(PT)。结果合成的蓝贻贝抗凝肽粗品的纯度为65.5%,经半制备纯化后的精肽纯度为95.5%;抗凝活性实验显示APTT、TT具有显著的延时作用(P<0.01)。结论固相合成的蓝贻贝抗凝肽具有抗凝活性。
OBJECTIVE To synthesize blue mussel Mytilus edulis anticoagulant peptide (MEAP) by solid-phase peptide synthesis and study its anticoagulant activity. Methods 2-CTC resin as carrier, Fmoc-protected amino acid as raw material, according to the sequence of blue mussel anticoagulant peptide from C-terminal to N-terminal coupling amino acid, after cleavage to obtain crude peptide, Peptides thrombin time (TT), activated partial thromboplastin time (APTT), prothrombin time (PT). Results The purity of the crude product was 65.5%. The purity of refined peptide was 95.5%. The anticoagulant activity showed that APTT and TT had a significant delayed effect (P <0.01). Conclusion Solid phase synthesis of blue mussel anticoagulant peptide has anticoagulant activity.