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目的 我们应用逆转录病毒构建了细胞因子IL—12表达载体,以研究基因修饰的肿瘤细胞的癌疫苗作用。方法 将其分别转染EL—4胸腺瘤细胞并研究了该基因导入细胞的抗肿瘤免疫效果。结果 当接种子EL—4/IL—12转染细胞后,在C57BL/6同系鼠中其基因导入细胞的肿瘤原性比较EL—4/Wt和EL—4/Neo组明显减少(P<0.01),在EL—4/IL—12被排斥后,体内试验中实验动物诱发了抗EL—4/Wt的系统性、保护性免疫。结论 这一研究结果提示应用IL—12进行血液肿瘤的治疗是有效的,在未来人类癌症的治疗中亦可有一定的应用前景。
OBJECTIVE: We used retroviruses to construct a cytokine IL-12 expression vector to study the role of cancer vaccines in genetically modified tumor cells. Methods Transfected EL-4 thymoma cells and studied the anti-tumor immunity effect of the gene-introduced cells. Results After transfected with EL-4/IL-12 cells, the tumorigenicity of the transfected cells in C57BL/6 syngeneic mice was significantly reduced compared with EL-4/Wt and EL-4/Neo groups (P<0.01). ) After the exclusion of EL-4/IL-12, the experimental animals in vivo induced a systemic and protective immunity against EL-4/Wt. Conclusions This study suggests that the use of IL-12 for the treatment of hematological neoplasms is effective and may have a certain application prospect in the future treatment of human cancers.