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目的探讨转录因子Twist及其上皮黏附分子E-cadherin在人胰腺癌表达的情况,及其与患者预后的相关性。方法采用免疫组织化学法分别检测60例胰腺癌组织和20例癌旁正常胰腺中Twist及E-cadherin的表达情况,分析Twist表达与临床病理学特性及预后的相关性。结果 Twist在胰腺癌组织中的表达明显强于癌旁正常胰腺组织(80.0%vs40.0%),而E-cadherin的表达减弱(50.0%vs100%),两者呈负相关(r=-0.1986,P<0.05)。Twist阳性表达与肿瘤位置、肿瘤浸润、淋巴组织转移和远处转移、pTNM分期有关(P<0.01),但与年龄、性别或分化程度无关(P>0.05)。Kaplarr-Meier生存分析显示,胰腺癌患者的生存率随Twist表达增强显著下降(Log-rank检验P<0.01)。结论胰腺癌中Twist的异常表达与肿瘤的侵袭、远处转移密切相关,且与E-cadherin的表达负相关,对患者术后生存时间有显著的影响,可能是评估胰腺癌预后的潜在指标。
Objective To investigate the expression of transcription factor Twist and its epithelial adhesion molecule E-cadherin in human pancreatic cancer and its relationship with the prognosis of patients. Methods The expressions of Twist and E-cadherin in 60 cases of pancreatic cancer and 20 cases of normal paraneoplastic tissues were detected by immunohistochemistry. The correlation between Twist expression and clinical pathological features and prognosis was analyzed. Results The expression of Twist in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues (80.0% vs 40.0%), while the expression of E-cadherin was weakened (50.0% vs 100%) (r = -0.1986 , P <0.05). Twist positive expression was related to tumor location, tumor infiltration, lymphoid tissue metastasis and distant metastasis, pTNM stage (P <0.01), but not with age, sex or differentiation (P> 0.05). Kaplan-Meier survival analysis showed that the survival rate of pancreatic cancer patients significantly decreased with Twist expression (Log-rank test P <0.01). Conclusions The abnormal expression of Twist in pancreatic cancer is closely related to the invasion and metastasis of tumor, and it is negatively correlated with the expression of E-cadherin. It has a significant effect on the postoperative survival time and may be a potential indicator to evaluate the prognosis of pancreatic cancer.