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纯化三次的日本毛虾、中国毛虾和中华假磷虾的Tm含量分别占其干重的0.86％,0.87％,和0.70％;SDS-PAGE测定两种毛虾Tm亚基分子量约3.3—3.6×10~4,磷虾的约3.2—3.5×10~4;等电聚焦分析出此三种动物Tm的pI约为pH5.0,在pH4.8或7.2的镁溶液中,三种Tm均能形成针状晶体,透射电镜下可见到约39nm横纹周期及若干细节;三种Tm均含有Glu,Asp、Lys、His,Arg、Ser、Thr、Gly、Ala、(Cys)_2、Val、Met、Ile、Leu、Tyr和Phe,但都未测出Pro和Trp;圆二色性测定指出,在远紫外区三种Tm均显示出峰型极为相似的典型α螺旋双负峰,峰极点分别落在222和208nm处,日本毛虾、中国毛虾和中华假磷虾Tm在208nm处的α螺旋度分别占整个肽链的76.6％,74.2％和61.6％。
The Tm contents of three purified Japanese shrimp, Chinese shrimp and Chinese pseudo-krill accounted for 0.86%, 0.87% and 0.70% of their dry weight respectively. The molecular weights of two shrimp Tm subunits were about 3.3-3.6 × 10 ~ 4, and krill were about 3.2-3.5 × 10 ~ 4. The isoelectric focusing analysis showed that the pI of Tm of these three animals was about pH5.0. In the magnesium solution of pH4.8 or 7.2, the three Tm Can form acicular crystals, the stripe cycle and some details about 39nm can be seen under transmission electron microscope; three Tm contain Glu, Asp, Lys, His, Arg, Ser, Thr, Gly, Ala, Met, Ile, Leu, Tyr and Phe, but none of Pro and Trp was detected. Circular dichroism measurements showed that all three Tm showed similar double-negative peaks of typical α-helix in extreme ultraviolet region. At 222 and 208 nm, respectively, the α-helicity of Tm at 208 nm for Japanese shrimp, Chinese shrimp and Sinorama krill accounted for 76.6%, 74.2% and 61.6% of the total peptide chain, respectively.