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目的观察脑损伤后神经细胞凋亡变化及黄体酮的保护作用。方法雄性SD大鼠随机分为假手术组、脑损伤组和黄体酮治疗组,大鼠脑损伤模型制作是利用改良的Feeney自由落体损伤装置。各组于伤后24、48、72 h取材。用免疫组织化学法观察大鼠皮质和海马CA1区半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达水平变化。结果脑损伤组的Caspase-3阳性细胞数在皮质和海马CA1区较假手术组显著增加,黄体酮治疗组与脑损伤组比较,Caspase-3阳性细胞数明显减少,差异有统计学意义(P<0.05)。结论黄体酮抑制脑损伤后Caspase-3的表达可能是黄体酮脑保护作用机制之一。
Objective To observe the changes of neuronal apoptosis and the protective effect of progesterone after brain injury. Methods Male Sprague-Dawley rats were randomly divided into sham operation group, brain injury group and progesterone treatment group. The model of rat brain injury was made by using the modified Feeney free-fall injury device. The groups were drawn 24, 48, 72 h after injury. The expression of Caspase-3 in cortex and hippocampus CA1 of rat was observed by immunohistochemistry. Results The number of Caspase-3 positive cells in brain injury group was significantly higher than that in sham operation group in cortex and hippocampal CA1 area, and the number of Caspase-3 positive cells in progesterone treatment group was significantly decreased compared with that in brain injury group (P <0.05). Conclusion Progesterone inhibits the expression of Caspase-3 after traumatic brain injury and may be one of the protective mechanisms of progesterone.