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从大豆品种‘垦农18’中克隆了一个CONSTANS-like基因,命名为GmCOL8。进化树分析表明它属于CONSTANS-like亚家族1的成员。mRNA表达分析显示,GmCOL8在短日下具有明显的生物钟节律性表达特性,其表达高峰出现在凌晨。GmCOL8主要在复叶中表达,表达高峰出现在开花时期。
A CONSTANS-like gene was cloned from soybean cultivar Kennong18 and named GmCOL8. Phylogenetic tree analysis showed that it belonged to CONSTANS-like subfamily 1. mRNA expression analysis showed that GmCOL8 had obvious circadian rhythmic expression characteristics in the short day and peaked in the early morning. GmCOL8 is mainly expressed in the compound leaf, the expression peak appears in the flowering period.