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目的观察慢性乙肝、肝硬变患者血清转化生长因子β１（ＴＧＦβ１）与血清肝纤维化指标和肝组织病理的关系．方法采用酶联免疫吸附试验（ＥＬＩＳＡ）测定７６例慢性乙肝、肝硬变患者（其中慢性轻度２０例、中度２０例、重度１８例、肝硬变１８例）血清ＴＧＦβ１．同时检测血清ＰＣⅢ，ＬＮ，ＨＡ．２９例作肝活检，行ＨＥ染色．结果慢性乙肝轻、中、重度及肝硬变患者血清ＴＧＦβ１水平（ｍｇ／Ｌ，１４４±５７，３５０±１０８，５０８±１３３，５７９±２２５）明显高于对照组（ｍｇ／Ｌ，９５±３４，Ｐ＜００１），按慢乙肝轻、中、重度、肝硬变依次升高（Ｐ＜００１或Ｐ＜００５）；ＴＧＦβ１与血清ＰＣⅢ，ＬＮ，ＨＡ呈正相关（Ｐ＜００１或Ｐ＜００５）；血清ＴＧＦβ１，ＰＣⅢ，ＬＮ，ＨＡ水平随着肝组织纤维化程度的加重而增长（Ｐ＜００１或Ｐ＜００５）．结论慢性乙型病毒性肝炎、肝硬变患者血清ＴＧＦβ１与肝纤维化的血清标志以及肝组织病理改变相一致．
Objective To observe the relationship between serum transforming growth factor-β1 (TGF-β1), serum markers of liver fibrosis and liver histopathology in patients with chronic hepatitis B and cirrhosis. Methods 76 patients with chronic hepatitis B and cirrhosis (20 with chronic mild, 20 with moderate, 18 with severe, 18 with cirrhosis) were assayed by enzyme-linked immunosorbent assay (ELISA) for serum TGF-β1. Simultaneous detection of serum PC Ⅲ, LN, HA. 29 cases of liver biopsy, HE staining. Results Serum levels of TGF-β1 in patients with mild, moderate, severe and cirrhosis of chronic hepatitis B were significantly higher than those in the control group (mg / L, 95 ± 108,350 ± 108,508 ± 133,579 ± 225) ± 34, P <001), according to chronic hepatitis B light, moderate and severe, cirrhosis in turn increased (P <0 01 or P <0 05); TGF β1 and serum PC Ⅲ, LN, HA was positive (P <001 or P <005). Serum levels of TGFβ1, PCⅢ, LN and HA increased with the degree of liver fibrosis (P <001 or P <005) . Conclusion Serum TGF-β1 in patients with chronic hepatitis B and cirrhosis is consistent with serum markers of liver fibrosis and pathological changes of liver tissue.