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目的研究多效生长因子(pleiotropin,PTN)在牙周炎治疗前后的表达情况。方法应用RT-PCR、原位杂交方法,利用雄性Wistar大鼠50只分为炎症组、恢复组和对照组分别检测PTN的表达情况。结果 RT-PCR半定量分析PTNmRNA在炎症组和恢复组两组中的表达P<0.01,有统计学意义的。PTNmRNA的阳性产物位于牙龈上皮、龈沟内上皮和结合上皮细胞胞浆中,呈棕黄色颗粒。炎症组中PTNmRNA的表达主要集中于棘细胞层和颗粒细胞层,表达量为弱阳性和中强阳性。恢复组中PTNmRNA的表达主要集中于棘细胞层和基底细胞层,表达量为强阳性和中强阳性。对照组中PTNmRNA的表达较少。RT-PCR与原位杂交检测结果基本一致(P<0.05)。结论 PTN同时具有致炎和促进组织修复的功能,但促进组织修复的作用更明显。
Objective To study the expression of pleiotropin (PTN) before and after periodontitis treatment. Methods Fifty male Wistar rats were divided into three groups according to RT-PCR and in situ hybridization. The recovery group and the control group were used to detect the expression of PTN. Results RT-PCR semi-quantitative analysis of PTNmRNA expression in the inflammatory group and recovery group P <0.01, statistically significant. The positive products of PTN mRNA were found in the gingival epithelium, the sulcus of gingival sulcus and the cytoplasm of conjunctival epithelial cells with brown granules. The expression of PTNmRNA in inflammatory group mainly concentrated in the spine cell layer and granular cell layer, the expression of weakly positive and moderately positive. In the recovery group, the expression of PTN mRNA mainly concentrated in the spine cell layer and the basal cell layer, and the expression levels were strongly positive and moderately strong. The control group, PTNmRNA expression less. RT-PCR and in situ hybridization results were basically the same (P <0.05). Conclusions PTN has the function of promoting inflammation and promoting tissue repair at the same time, but the effect of promoting tissue repair is more obvious.