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为在兼顾生物量的基础上提高液体发酵中三萜的百分含量,首先通过单因子试验筛选出最佳的碳源、氮源、无机盐,然后采用均匀设计(UD)对其培养基配比和培养条件进行优化试验,建立适当的数学模型,并对模型进行验证确定优化条件.单因子试验结果表明玉米淀粉既能促进菌丝体的生长,又能促进三萜的合成;麸皮虽然对菌体生长略有不利,但是对三萜的积累作用特别显著;硫酸镁对菌丝体生长效果不显著,但是对三萜的合成比较有利.均匀设计试验结果表明,菌丝体干重最大的培养条件和三萜的最优培养条件相差甚远.菌丝体干重的最优培养条件为:1 L培养液中玉米淀粉47 g,麸皮47 g,硫酸镁0.5 g,初始pH 3.0,培养7 d;三萜最佳培养基配方为:1 L培养液中玉米淀粉20 g,麸皮20 g,硫酸镁1.85 g,初始pH 3.0,培养16 d.菌丝体干重在最优条件下能达到15.58(±0.37)g L-1;三萜百分含量在最优培养条件下高达6.04(±0.03)%,三萜的百分含量比基础发酵结果和报道的最高发酵结果3.18%提高了90%,因此,均匀设计法能有效优化液体发酵培养条件
In order to improve the percentage of triterpenes in liquid fermentation based on both biomass, the best carbon source, nitrogen source and inorganic salt were screened out by single factor test. Then, UD Optimization and culture conditions to establish an appropriate mathematical model and validation of the model to determine the optimal conditions.Factor experiments showed that corn starch can promote the growth of mycelium, but also promote the synthesis of triterpenoids; although bran The growth of mycelium is slightly unfavorable, but the accumulation of triterpenoids is particularly significant; the effect of magnesium sulfate on the growth of mycelium is not significant, but the synthesis of triterpene is more favorable.Uniform design test results show that the mycelium dry weight is the largest Culture conditions and triterpene optimum culture conditions are far cry.The optimal culture conditions for the mycelium dry weight: 1 L cornstarch 47 g, bran 47 g, magnesium sulfate 0.5 g, initial pH 3.0 , And cultured for 7 days.The best medium for triterpene was 20 g of corn starch, 20 g of bran and 1.85 g of magnesium sulfate in 1 L of culture medium, initial pH was 3.0 and cultured for 16 days.The dry weight of mycelia was the best Under the conditions to reach 15.58 (± 0.37) g L-1; triterpene percentage in the optimal training Up to 6.04 (± 0.03)% under raising conditions, the percentage of triterpenes is increased by 90% compared to the basal fermentation results and the reported maximum fermentation result of 3.18%. Therefore, the uniform design method can effectively optimize the liquid fermentation culture conditions