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目的观察慢性铅暴露对大鼠海马齿状回长时程增强(Long-Term Potentiation,LTP)和蛋白激酶C(Protein Kinase C,PKC)表达的影响及可能的机制。方法 24只大鼠随机分为2组,即对照组和铅暴露组。铅暴露组每天饲以醋酸铅水溶液(0.05g/L),对照组供以蒸馏水。在28 d后,用在体细胞外脑电记录方法比较强直刺激前后海马齿状回群体峰电位(Population Spike,PS)幅度,Westernblot方法检测海马PKC的表达。结果铅暴露组强直刺激后平均PS幅度增幅为(126.13±1.94)%,显著低于对照组的(183.79±1.35)%。海马齿状回PKC表达量减少。结论铅暴露明显抑制了大鼠海马齿状回LTP,并且抑制海马PKC的表达,从而导致学习记忆功能的下降。
Objective To observe the effect of chronic lead exposure on the expression of LTP and PKC in rat hippocampus and its possible mechanism. Methods Twenty-four rats were randomly divided into two groups: control group and lead exposure group. Lead exposure group fed with lead acetate solution (0.05g / L), the control group for distilled water. After 28 days, the amplitude of hippocampal dentate gyrus (Population Spike, PS) was measured by extracellular EEG recording before and after tetanic stimulation. The expression of PKC in hippocampus was detected by Western blot. Results The average PS amplitude of lead exposure group was (126.13 ± 1.94)%, significantly lower than that of control group (183.79 ± 1.35)%. Decreased PKC expression in hippocampal dentate gyrus. Conclusion Lead exposure significantly inhibits hippocampal dentate gyrus LTP and inhibits the expression of PKC in the hippocampus, leading to the decline of learning and memory functions.