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以凋亡诱导剂喜树碱影响下的ＨＬ－６０细胞为检测对象，对以流式细胞仪为基础建立的３种细胞凋亡定量分析方法，进行了敏感性和特异性比较，结果：３种方法对凋亡均有定性和定量作用，均能同时提供细胞周期方面的信息。加入喜树碱（ＣＡＭ）１２０ｍｉｎ后，低分子量ＤＮＡ抽提后荧光染料（ＰＩ）染色法（Ｓｕｂ－Ｇ１法）和ＴｄＴ酶探测ＤＮＡ断端方法（ＴｄＴ法）均可检测到凋亡细胞；而ＤＮＡ变性后Ｙ啶橙染色法（ＡＯ法）在加入ＣＡＭ１００ｍｉｎ后，就能够证实细胞凋亡的存在。ＴｄＴ法所显示的检测结果最清晰、直观。Ｓｕｂ－Ｇ１法操作最简便、迅速，费用最低。结论：ＡＯ法敏感性好；ＴｄＴ法更适合于细胞凋亡的细胞周期特异性分析；Ｓｕｂ－Ｇ１法最具实用性。
To detect HL-60 cells under the influence of apoptosis inducer camptothecin, we compared the sensitivity and specificity of three kinds of apoptosis quantitative analysis methods established on the basis of flow cytometry. Results: 3 Methods of apoptosis both qualitative and quantitative effects, both can provide the cell cycle information. After adding camptothecin (CAM) for 120min, apoptotic cells could be detected by PI staining (Sub-G1 method) and TdT DNA sequencing (TdT method) after low molecular weight DNA extraction. DNA denaturation Y-orange staining (AO method) After adding CAM100min, we can confirm the existence of apoptosis. TdT method shows the most clear and intuitive test results. Sub-G1 method is the easiest, fastest and lowest cost. Conclusion: AO method is sensitive; TdT method is more suitable for cell cycle-specific analysis of apoptosis; Sub-G1 method is the most practical.