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实验证明在基础芳烃羟化酶活性很低的人FL细胞系中该酶活性可因去甲肾上腺素,β-萘黄酮及3-甲基胆蒽的诱导而明显升高。基础及诱导的芳烃羟化酶具有细胞色素P-448依存性混合功能氧化酶的性,即依赖于还原型辅酶Ⅱ,对CO敏感和可被7,8-苯黄酮抑制。在诱导剂自培养基中撤除后,至少在24～36小时内芳烃羟化酶活性仍维持在较高水平。应用芳烃羟化酶经过诱导的细胞,用非程序性DNA合成(UCS)试验捡测前诱变剂/前致癌剂证明,黄曲霉毒素B_1、3-甲基胆蒽及苯并(a)芘等前诱变剂/前致癌剂可在经β-萘黄酮诱导的细胞中诱发很明显的UDS反应,而在未经诱导的细胞中为阴性或仅有轻微反应。上述结果表明这种新设计对前诱变剂/前致癌剂的检测是可行的,但尚需进一步研究以完善之。
It has been experimentally demonstrated that this enzyme activity can be significantly increased by the induction of norepinephrine, β-naphthoflavone and 3-methylcholanthrene in a human FL cell line in which the basal aromatic hydrocarbon hydroxylase activity is low. The basal and induced aromatase hydroxylases have the properties of a mixed functional oxidase that is cytochrome P-448-dependent, ie, dependent on reduced coenzyme II, which is sensitive to CO and can be inhibited by 7,8-benzoflavone. After the inducer was removed from the medium, the aromatase hydroxylase activity was maintained at a high level for at least 24 to 36 hours. Using aromatase hydroxylase-induced cells, non-programmer DNA synthesis (UCS) tests of mutagen / procarcinogen agents before and / or progenitor tests showed that aflatoxins B_1,3-methylcholanthrene and benzo (a) pyrene Equal pre-mutagenic / pro-carcinogenic agents can elicit a pronounced UDS response in cells induced by β-naphthoflavone but negative or only mild responses in non-induced cells. The above results show that the new design of pre-mutagenic agent / procarcinogen detection is feasible, but further research is needed to improve it.