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目的:建立同时测定畲药食凉茶中三种黄酮类成分含量的方法。方法:采用高效液相色谱法测定,色谱柱为Agilent Zorbax SB-C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为1.0 ml·min-1,柱温30℃;检测波长360 nm。结果:芦丁、槲皮素和山柰酚的线性范围分别为0.040 9~1.637 0 mg·ml-1(r=0.999 2),0.44~88.00μg·ml-1(r=0.999 8),0.41~77.63μg·ml-1(r=0.999 2);平均加样回收率分别为99.35%(RSD=1.64%),101.14%(RSD=1.88%),99.69%(RSD=1.92%)。结论:该方法简便易行、快速准确、重复性好,可用于同时测定食凉茶中的芦丁、槲皮素和山柰酚的含量。
Objective: To establish a method for simultaneous determination of three flavonoids in Her Medicine Herbal Medicine. Methods: The HPLC column was Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution. The flow rate was 1.0 ml · min -1, column temperature 30 ℃; detection wavelength of 360 nm. RESULTS: The linear ranges of rutin, quercetin and kaempferol were 0.040 9-1.637 0 mg · ml-1 (r = 0.999 2), 0.44-88.00 μg · ml-1 (r = 0.999 8), 0.41 ~ 77.63μg · ml-1 (r = 0.999 2). The average recoveries were 99.35% (RSD = 1.64%), 101.14% (RSD = 1.88%) and 99.69% (RSD = 1.92%) respectively. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used for the simultaneous determination of rutin, quercetin and kaempferol in the herbal tea.