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目的 研究库司托啶 (Custodiol)与异搏定 (Verapamil)联合应用对低温保存大鼠肝脏的保护作用。方法 将 40只Wistar大白鼠制作肝离体灌注模型 ,库司托啶和异搏定作肝脏灌注并置于 4℃保存 6、2 4和 48h后观察肝窦内皮细胞死亡率、肝功能以及肝细胞形态的变化。结果 库司托啶和异搏定灌注组明显优于对照组 ,保存 2 4h ,库司托啶 +异搏定 (CVA)组、库司托啶组的肝窦内皮细胞死亡率分别为 30 .2 9%和 36 .36 % ,低于异搏定组 (5 7.5 3 % )和林格氏液组 (6 1.5 5 % ) ,其差异有显著性 (P <0 .0 5 )。保存 48h ,CVA组 (4 9.46 % )、库司托啶组 (5 7.45 % )与异搏定组 (78.79% )、林格氏液组 (89.2 1% )之间的差异增大 ,其差异有非常显著性 (P <0 .0 0 1)。两者联合应用优于单独应用。结论 库司托啶和异搏定联合应用对离体大鼠肝脏有明显的保护作用
Objective To study the protective effects of Custodiol combined with Verapamil on rat liver after cryopreservation. Methods Forty Wistar rats were used to establish the model of hepatic ex vivo perfusion, and were treated with streptozotocin and verapamil for liver perfusion. After 4, 4 and 48 hours of storage, hepatic sinusoidal endothelial cell death rate, liver function and liver function Changes in cell morphology. Results The results showed that the serum levels of stutortol and verapamil in the perfusion group were significantly higher than those in the control group. After 24 h of storage, the hepatic sinusoidal endothelial cell death rates were 30 in the kututilide + verapamil (CVA) group and the kutuzol group. 2 9% and 36.36% respectively, which were lower than that of the patients treated with verapamil (7.5 7.5%) and the Ringer’s solution (6 1.55%) (P <0.05). After 48h of storage, the difference between CVA group (4 9.46%), kilotuidine group (5 7.45%) and verapamil group (78.79%) and Ringer’s solution group (89.2 1%) increased There was a significant difference (P <0.001). The combination of both is better than single application. Conclusion The combination of reserpine and verapamil has a significant protective effect on isolated rat liver