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目的研究大肠癌组织ｎｍ２３ＨＩ，ｐ５３，ＰＣＮＡ的表达意义及其与肿瘤浸润转移的关系．方法１９９１年３月～１９９６年５月采用免疫组织化学方法检测７４例（男３９例，女３５例，平均年龄５３２岁，术前未做化疗及放疗）大肠癌组织（石蜡切片，厚４μｍ）中ｎｍ２３Ｈ１，ｐ５３，ＰＣＮＡ以及Ⅳ型胶原的表达情况．结果大肠癌ｎｍ２３Ｈ１，ｐ５３，ＰＣＮＡ的阳性率分别为７１６％、５２７％和８１１％．ｎｍ２３Ｈ１低表达与淋巴结转移有关（Ｐ＜００５），在Ⅳ型胶原表达不同的肠癌中ｎｍ２３－Ｈ１的表达无明显差异；ｐ５３和ＰＣＮＡ过表达与浸润程度和淋巴结转移有关（Ｐ＜００５）．结论ｎｍ２３ＨＩ低表达可作为预测大肠癌转移的较好指标．ｐ５３过表达在浸润转移过程及细胞增殖中均起重要作用．
Objective To investigate the expression of nm23-HI, p53 and PCNA in colorectal cancer tissue and its relationship with tumor invasion and metastasis. METHODS: From March 1991 to May 1996, immunohistochemical methods were used to detect 74 cases (39 males and 35 females, mean age 53 years and 2 years old, no chemotherapy and radiotherapy before surgery). Colorectal cancer tissues (paraffin sections, thick Expression of nm23-H1, p53, PCNA and type IV collagen in 4 μm). Results The positive rates of nm23-H1, p53 and PCNA in colorectal cancer were 71.6%, 52.7% and 81.1%, respectively. The low expression of nm23H1 was associated with lymph node metastasis (P<005). There was no significant difference in the expression of nm23-H1 between different types of colorectal cancers. The overexpression of p53 and PCNA was related to the degree of invasion and lymph node metastasis. <005). Conclusion The low expression of nm23-HI can be used as a better predictor of colorectal cancer metastasis. Overexpression of p53 plays an important role in the process of infiltration and cell proliferation.