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目的探讨N-乙酰-L-色氨酸(L-NAT)对大鼠肝脏缺血再灌注中肠损伤的保护作用。方法将健康成年雄性SD大鼠24只分为假手术组(Sham组)、缺血再灌注组(IR组)和缺血再灌注加L-NAT组(IR+L-NAT组)。用夹闭肝中叶和左叶肝蒂分支的方法制作肝缺血再灌注模型,用苏木素-伊红(HE)染色法观察小肠组织的形态学结构,用免疫组织化学染色观察激活型Caspase-3、Bax、Bcl-2的表达。结果 (1)IR组小肠绒毛结构破坏,肠黏膜充血脱落,上皮细胞变性坏死,出现炎症细胞浸润;LNAT可使之减轻。(2)免疫组织化学染色显示,与Sham组相比,IR组激活型Caspase-3、Bcl-2和bax的表达升高,L-NAT干预后,Caspase-3和Bax的表达下降,而Bcl-2的表达进一步升高。结论 L-NAT可抑制肝脏缺血再灌注损伤引起的小肠上皮细胞凋亡,减轻小肠上皮细胞损伤。
Objective To investigate the protective effect of N-acetyl-L-tryptophan (L-NAT) on midgut injury induced by hepatic ischemia-reperfusion in rats. Methods Twenty-four healthy adult male Sprague-Dawley rats were randomly divided into Sham group, IR group, IR-L-NAT group and IR + L-NAT group. The model of hepatic ischemia-reperfusion was established by clipping the middle and middle hepatic pedicle branches. The morphological changes of intestinal tissue were observed by hematoxylin and eosin (HE) staining. The expression of activated Caspase-3 , Bax, Bcl-2 expression. Results (1) The structure of villus in IR group was destroyed, the intestinal mucosa was exfoliated, the epithelial cells were degenerated and necrotic, inflammatory cells infiltrated; LNAT could reduce it. (2) Immunohistochemical staining showed that compared with Sham group, the expressions of activated Caspase-3, Bcl-2 and bax were increased in IR group, while the expression of Caspase-3 and Bax was decreased in L group -2 expression further increased. Conclusion L-NAT can inhibit intestinal ischemia-reperfusion injury-induced intestinal epithelial cell apoptosis, reduce intestinal epithelial cell injury.