论文部分内容阅读
目的观察胰岛素治疗对糖尿病大鼠学习记忆功能障碍及大脑皮层、海马内生长抑素(SS)、血管活性肠肽(VIP)含量变化的作用。方法采用链脲菌素制备雄性Wistar大鼠糖尿病模型,设正常对照组、胰岛素治疗糖尿病组、未治疗糖尿病组。3个月后采用T型水迷宫试验测定大鼠学习记忆功能,采用放免法测定皮层额叶、海马区脑组织SS、VIP含量。结果与正常对照组比较,未治疗糖尿病大鼠游迷宫时间显著延长、正确次数显著减少,额叶皮层、海马区脑组织SS含量显著降低,VIP含量无显著变化;胰岛素治疗组大鼠上述变化无显著意义。结论糖尿病引起大鼠学习记忆功能障碍和SS下降;SS下降与学习记忆功能障碍有关;早期胰岛素治疗可使学习记忆功能和SS含量恢复正常。
Objective To observe the effects of insulin therapy on the learning and memory dysfunction and the content of somatostatin (SS) and vasoactive intestinal peptide (VIP) in the cerebral cortex and hippocampus of diabetic rats. Methods The diabetic model of male Wistar rats was established by streptozotocin. Normal control group, insulin-treated diabetic group and untreated diabetic group were established. Three months later, the learning and memory function of rats was measured by T-type water maze test. The contents of SS and VIP in the frontal cortex and hippocampus were determined by radioimmunoassay. Results Compared with the normal control group, the untreated diabetic rats maze time was significantly prolonged, the correct number was significantly reduced, the frontal cortex, hippocampus brain tissue SS content was significantly reduced, VIP content was no significant change; insulin-treated rats did not change the above Significant. Conclusions Diabetes mellitus can cause learning and memory impairment and SS decline in rats. SS decline is associated with learning and memory dysfunction. Early insulin therapy can restore learning and memory function and SS content.