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目的探讨血管内皮生长因子C(VEGF-C)和淋巴管内皮细胞透明质酸受体1(LYVE-1)在低位直肠癌组织中的表达及意义。方法应用免疫组化法检测92例低位直肠癌组织VEGF-C及LYVE-1的表达水平及其与临床病理因素的关系。结果(1)淋巴结转移组VEGF-C的阳性表达率为62.1%,无淋巴结转移组阳性表达率为37.9%。LYVE-1可计算微淋巴管密度(LMVD),其阳性产物表达主要集中于癌组织边缘和正常黏膜的交界处。直肠癌组织中VEGF-C表达阴性者,LMVD是(1.95±0.56)个/HP;而VEGF-C表达阳性者,LMVD是(4.98±1.76)个/HP,两者差异有统计学意义(P<0.05)。VEGF-C表达阳性者LYVE-1的表达率是47.9%。(2)LMVD与直肠癌的分化程度,TNM分期,淋巴结转移及远处转移有关;这4个指标各分组间差异有统计学意义(P<0.01或P<0.05)。结论VEGF-C和LYVE-1在直肠癌转移淋巴结中均呈高表达,可能有协同效应,共同促进直肠癌的淋巴转移。淋巴管生成在直肠癌的发展中起着重要作用,LMVD可作为直肠癌进展程度的重要指标。
Objective To investigate the expression and significance of vascular endothelial growth factor C (VEGF-C) and lymphatic endothelial cell hyaluronan receptor 1 (LYVE-1) in low rectal cancer. Methods The expression of VEGF-C and LYVE-1 in 92 cases of low rectal cancer and their relationship with clinicopathological parameters were detected by immunohistochemistry. Results (1) The positive rate of VEGF-C expression was 62.1% in lymph node metastasis group and 37.9% in non-lymph node metastasis group. LYVE-1 can be calculated lymphatic vessel density (LMVD), the expression of its positive product mainly in the edge of the cancer tissue and normal mucosal junction. The positive rate of LMVD was (1.95 ± 0.56) / HP in patients with negative VEGF-C expression and the positive rate of LMVD was (4.98 ± 1.76) / HP in patients with positive VEGF-C expression (P <0.05). The expression rate of LYVE-1 with positive VEGF-C expression was 47.9%. (2) LMVD was correlated with the degree of differentiation, TNM stage, lymph node metastasis and distant metastasis in rectal cancer. There was significant difference among the four groups (P <0.01 or P <0.05). Conclusions Both VEGF-C and LYVE-1 are highly expressed in metastatic lymph nodes of rectal cancer, which may have synergistic effect to promote lymphatic metastasis of rectal cancer. Lymphangiogenesis plays an important role in the development of rectal cancer. LMVD can be used as an important indicator of the progression of rectal cancer.