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目的观察三七总皂甙对大鼠脑缺血再灌注损伤后p53蛋白表达的影响,探索三七总皂甙神经保护作用的分子机制。方法采用SD大鼠局灶性脑缺血再灌注模型(transientmiddle cerebral arteryocclusion,MCAO),将大鼠随机分组,各组按实验设计给药造模,分时点对各组动物造模后神经功能缺陷进行评分,应用免疫组织化学法研究缺血再灌注损伤脑组织凋亡相关蛋白p53的表达情况。结果PNS处理各组缺血再灌注后神经功能缺陷评分和脑组织p53蛋白表达均明显少于缺血再灌注组(P<0.05)。结论三七总皂甙能减轻脑组织的缺血再灌注损伤,改善神经功能缺失。其作用机制可能与其抑制脑组织p53蛋白表达有关。
Objective To observe the effect of panax notoginseng saponins on the expression of p53 protein in rats with cerebral ischemia reperfusion injury, and to explore the molecular mechanism of the neuroprotective effect of panax notoginseng saponins. Methods SD rats were subjected to focal cerebral ischemia-reperfusion model (transient middle cerebral artery occlusion, MCAO). The rats were randomly divided into groups. Each group was given an experimentally designed drug delivery model, and neurological function was modeled at each time point for each group of animals. Defects were scored and immunohistochemistry was used to study the expression of apoptosis-related protein p53 in brain tissue following ischemia-reperfusion injury. Results The scores of neurological deficits and the expression of p53 protein in brain tissue were significantly lower in PNS-treated groups than in ischemia-reperfusion group (P<0.05). Conclusion Panax Notoginseng can relieve cerebral ischemia-reperfusion injury and improve neurological deficit. The mechanism may be related to its inhibition of p53 protein expression in brain tissue.