论文部分内容阅读
目的探讨艾滋病病毒(HIV)感染对ELISA试剂检测静脉吸毒人员丙型肝炎病毒(HCV)抗体的影响,为HIV、HCV混合感染检测策略的提出和修改提供理论依据。方法按是否感染HIV分组对两种抗-HCVELISA试剂检测反应强度(S/CO)≥1的分布进行对比,采用SPSS 15.0软件分析。结果两组样本经两种试剂检测反应强度(S/CO)的构成差异有统计学意义(P<0.05),HIV阳性组HCV抗体检测低反应强度的构成高于HIV阴性组(P<0.05)。对科华试剂检测为低反应强度的标本,与用Ortho试剂检测结果的一致性在两组间差异有统计学意义(P<0.05)。结论是否感染HIV对科华HCV抗体ELISA试剂及Ortho HCV 3.0 ELISA试剂反应强度均有影响。HIV感染者可能出现HCV抗体ELISA检测不确定或假阴性反应,弱阳性反应标本可增加假阳性可能。
Objective To investigate the influence of HIV infection on the detection of hepatitis C virus (HCV) antibodies by ELISA in patients with intravenous drug addicts, and to provide a theoretical basis for the detection and modification of HIV and HCV mixed infections. Methods The distributions of response intensity (S / CO) ≥1 of two anti-HCV ELISA reagents were compared according to whether they were infected with HIV or not and analyzed by SPSS 15.0 software. Results There was a significant difference in the composition of S / CO between the two samples (P <0.05). The HIV antibody positive rate of HCV antibody detection in HIV positive group was higher than that of HIV negative group (P <0.05) . The difference between the two groups was statistically significant (P <0.05). The consistency of the results of Kehua reagent with Ortho reagent was statistically significant (P <0.05). Conclusion Whether HIV infection affects the reaction intensity of Kehua HCV antibody ELISA reagent and Ortho HCV 3.0 ELISA reagent. HCV infection may occur HCV antibody ELISA detection of uncertain or false negative reactions, weak positive samples may increase false positives.